The proposed European Super League is the result of “selfishness and greed,” says Uefa vice-president Fernando Gomes.
It was revealed last week that talks had taken place over the creation of a new £4.6bn competition.
Outgoing Barcelona president Josep Maria Bartomeu said the club had begun proceedings to join the league.
“It violates all principles of sporting merit. It would be a self-proclaimed privileged club,” Gomes said.
The European Super League would involve the top sides from across the continent, although discussions are at an early stage.
In his resignation speech, Bartomeu said Barca had started proceedings to help their financial future.
However, Gomes said it would have “no passage of support” in his native Portugal.
“It deserves my refusal because the world is currently experiencing its greatest challenge, at least for the last century, and the last thing it needs is the exacerbation of selfishness and greed,” he added.
“In my opinion all governing bodies should refuse it in a very clear way.”